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Microfluidic-Based Approaches for Foodborne Pathogen
Detection
Xihong Zhao
1Research Center for Environmental Ecology and
Engineering, Key Laboratory for Green substance Process of Ministry of
Education,
Key Laboratory for Hubei Novel Reactor & Green
Chemical Technology, School of Environmental Ecology and Biological
Engineering, Wuhan Institute of Technology, Wuhan 430205, China;
moc.361@53320979231
Mei Li
1Research Center for Environmental Ecology and
Engineering, Key Laboratory for Green Chemical development of Ministry of
Education,
Key Laboratory
for Hubei Novel Reactor & Green Chemical Technology, School of
Environmental Ecology and Biological Engineering, Wuhan Institute of
Technology, Wuhan 430205, China; moc.361@53320979231
Yao Liu
2School of Pharmacy and Food Science, Zhuhai College
of Jilin University, Zhuhai 519041, China
Abstract
Food protection is of obvious importance, but there
are common troubles because of foodborne pathogens that threaten the protection
and health of humans international. Although the maximum traditional approach
for detecting micro organism is the plate counting approach, it takes almost 3
to seven days to get the bacterial consequences for the detection.
Additionally, there are many present technologies for correct determination of
pathogens,
together with polymerase chain reaction (PCR), enzyme
connected immunosorbent assay (ELISA), or loop-mediated isothermal
amplification (LAMP), but they're not suitable for timely and fast on-web site
detection due to time-consuming pretreatment, complicated operations and false
tremendous effects.
Therefore, an
urgent goal remains to determine how to fast and effectively prevent and
manipulate the prevalence of foodborne illnesses which are dangerous to people.
As an alternative, microfluidic devices with miniaturization, portability and
low cost had been brought for pathogen detection. In precise, using
microfluidic technology is a promising path of research for this motive.
Herein, this text systematically reviews the use of microfluidic generation for
the fast and sensitive detection of foodborne pathogens. First, microfluidic
technology is brought, together with the fundamental concepts, background, and
the pros and cons of different beginning materials for particular applications.
Next, the applications and issues of microfluidics for
the detection of pathogens are mentioned. The present day reputation and
one-of-a-kind packages of microfluidic-based totally technologies to
distinguish and identify foodborne pathogens are defined in element. Finally,
destiny trends of microfluidics in food protection are discussed to offer the
necessary foundation for destiny studies efforts.
1. Introduction
With the rapid improvement of the economic system and the non-stop improvement of dwelling situations, humans today are paying increasingly more interest to fitness problems. At the same time, whether or not food is safe or now not is likewise carefully related to people’s fitness, therefore, it is also very critical to make certain the safety of meals.
Unfortunately, human beings on occasion unconsciously consume a few foods which
might be harmful to the body in their daily lives, as an instance, food
contaminated via pathogens [1,2]. If humans consume food containing foodborne
pathogens which includes Staphylococcus aureus, Salmonella, and Escherichia
coli O157:H7, they will go through vomiting or even dying,
triggering
patron panic . According to the records of Parisi et al. 1 / 4 of the world’s
humans are at better threat of foodborne ailments due to the modern-day
inefficient detection generation of bacteria, the imperfect meals supervision
system and excessive-pace economic development . Overall, new techniques must
be implemented to enhance food protection.
Foodborne illnesses are resulting from pathogens or their pollutants whilst they are contained in meals or water. Pathogens inflicting foodborne illnesses include micro organism, viruses, fungi, and parasites . For example, humans inflamed by way of pathogenic Escherichia coli (E. Coli) regularly experience extreme diarrhea, and there are almost 1.7 billion cases of diarrhoea each yr inside the international